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American Society of Microbiology ABMM Sample Questions:

1. A microbiology laboratory receives a blood culture from a patient with suspected bacterial endocarditis. After
24 hours of incubation, Gram-positive cocci in clusters are observed on the Gram stain. Catalase test is positive, and coagulase test is negative. Further testing reveals susceptibility to novobiocin. The MOST likely organism is:

A) Staphylococcus lugdunensis
B) Staphylococcus aureus
C) Staphylococcus epidermidis
D) Staphylococcus saprophyticus

2. A clinical microbiology laboratory is performing antimicrobial susceptibility testing on a Streptococcus pneumoniae isolate using broth microdilution. The lowest concentration of antibiotic that inhibits visible growth of the organism is reported as the:

A) Minimum inhibitory concentration (MIC)
B) Breakpoint
C) Zone diameter
D) Minimum bactericidal concentration (MBC)

3. A patient with a history of travel to a developing country presents with chronic diarrhea, abdominal pain, and weight loss. Stool examination reveals the presence of cysts containing four nuclei and chromatoidal bars with rounded ends. The MOST likely causative organism is:

A) Cryptosporidium parvum
B) Giardia lamblia
C) Entamoeba histolytica
D) Cyclospora cayetanensis

4. A clinical microbiology laboratory is investigating a potential outbreak of Legionella pneumophila associated with a hospital's water system. The MOST appropriate environmental samples to collect for culture and comparison to clinical isolates would be:

A) Surface swabs from frequently touched objects.
B) Sputum samples from asymptomatic healthcare workers.
C) Air samples from patient rooms and common areas.
D) Water samples from showers, faucets, and cooling towers.

5. A Klebsiella pneumoniae isolate from a bloodstream infection shows the following susceptibility profile:
Meropenem (MIC = 8 µg/mL, R), Imipenem (MIC = 16 µg/mL, R), Ertapenem (MIC = 4 µg/mL, R), Ceftazidime/avibactam (MIC = 1 µg/mL, S), Aztreonam (MIC = 32 µg/mL, R). Which resistance mechanism is most strongly suggested by this pattern?

A) Metallo-beta-lactamase (MBL) production
B) Klebsiella pneumoniae Carbapenemase (KPC) production
C) Extended-Spectrum Beta-Lactamase (ESBL) production alone
D) AmpC beta-lactamase hyperproduction coupled with porin loss

Solutions: